CCET USER SERVICES

APPLY TO USE CCET

CRYO-FIB-SEM

The center operates an Aquilos-2 Cryo-FIB SEM for preparing lammela of various samples including cells and tissues

CLEM

Correlative light and electron microscopy (CLEM) is implemented by the facilty using the Leica Thunder CLEM system 

CRYO-PREP

The center operates a Vitrobot Mark V, a CrysoSol Vitrojet and a Wohlwend Compact 2 HPF  for rapid vitrification of cells

 SCREENING

Samples for high resolution data collection are screened on-site using the available F30 and F20 FEG electron microscopes

DATA COLLECTION

The center can collect initial tomograms using the on-site F30 and F20 FEG electron microscopes housed in the adjacent EM services facility

DATA ANALYSIS

The center maintains two GPU workstations each comprised of 4 1080 GPU processsors and one terabyte of local RAM and 12 terabytes of storage

USER TRAINING

The center conducts annual workshops as well as one on one tutorials to train users in every aspect of cryoET from sample preparation to data analysis

METHOD DEV OPS

Part of the centers mandate is to develop improved methods for sample preparation and we are particularly focused on time resolved cryoET